Essentials II – Essentials of confocal microscopy
Organizer: Manuel Gunkel, Aliaksandr Halavatyi
Registration for EMBL staff only
The course covers the essential knowledge needed to conduct confocal microscopy-based experiments and takes place in the ALMF on January 28th (9:30 am to 4:30 pm).
The prerequisite for attending this course is the knowledge covered in the Essentials I course. The microscopy knowledge covered in this is mandatory for introductions into ALMF Confocal-microscopes.
For preparation of the course you will get a short list of videos covering the basics.
Topics covered include:
- Confocal principle
- Optical sectioning
- Confocal Lightpath
- Scanning
- Pinhole size
- Lateral and axial resolution
- 3D data acquisition with confocals
- Saturation and offset
- SNR Averaging/Accumulation, Voltage, laser power
- Refractive index and spherical aberrations
- Correcting for z-distortion
- Multicolor imaging
- Getting a transmitted light image with the confocal microscope
- Sample types