Essentials I – Essentials of Widefield-Microscopy

Organizer: Stefan Terjung, Beate Neumann, Marko Lampe, Manuel Gunkel and Christian Tischer

Registration for EMBL staff only

The course is a beginners course with focus on teaching the essential knowledge needed to conduct a widefield microscopy-based experiment, to generate reliable data and to inspect and handle the acquired images. It will take place as a two day event and consists of a practical session on January 23rd (10 am – 5 pm) in the ALMF and an image handling and inspection session on Wednesday January 30th (2 – 5 pm) together with CBA.
Knowledge of this course will be the basis for the Essentials in Microscopy II (Confocal Microscopy) course.

For preparation of the course you will get a short list of videos covering the basics as well as a self-test questionaire

Topics covered include

• Transmission light path and Köhler illumination

• Fluorescence microscopy, filters and how to find good filter fluorophore combinations

• Light sources, Microscope objectives (magnification, resolution and brightness), Cover slips

• exposure time, bit depth and LUT, background level, remarks on sample preparation

Data handling and ispection topics:

Using ImageJ, we will cover the following topics related to 3D image data:
• Opening images
• Image content and inspection
• Image calibration
• Image data types
• Image file formats

Bookings