[ALMF] Essentials II – Essentials of confocal microscopy

almf   2025-01-21   Comments Off on [ALMF] Essentials II – Essentials of confocal microscopy

Date/Time
Date(s) - 2025-01-21
All Day

Essentials II – Essentials of confocal microscopy

Organizer: Manuel Gunkel, Aliaksandr Halavatyi

Registration for EMBL staff only

The course covers the essential knowledge needed to conduct confocal microscopy-based experiments and takes place in the ALMF on January 21st(9:30 am to 4:30 pm).
The prerequisite for attending this course is the knowledge covered in the Essentials I course. The microscopy knowledge covered in this is mandatory for introductions into ALMF Confocal-microscopes.

For preparation of the course you will get a short list of videos covering the basics.

Topics covered include:

  • Confocal principle
  • Optical sectioning
  • Confocal Lightpath
  • Scanning
  • Pinhole size
  • Lateral and axial resolution
  • 3D data acquisition with confocals
  • Saturation and offset
  • SNR Averaging/Accumulation, Voltage, laser power
  • Refractive index and spherical aberrations
  • Correcting for z-distortion
  • Multicolor imaging
  • Getting a transmitted light image with the confocal microscope
  • Sample types

Bookings

This event is fully booked.