[ALMF] Essentials I – Essentials of Widefield-Microscopy (online)

almf   2021-01-12   Comments Off on [ALMF] Essentials I – Essentials of Widefield-Microscopy (online)

Date(s) - 2021-01-12
10:00 CET - 17:00 CET

Essentials I – Essentials of Widefield-Microscopy (online)

Organizer: Sabine Reither, Marko Lampe and Lucas Schuetz

Registration for EMBL staff only

The course is a beginners course with focus on teaching the essential knowledge needed to conduct a widefield microscopy-based experiment and to generate reliable data. Knowledge of this course will be the basis for the Essentials in Microscopy II (Confocal Microscopy) course.

Please be aware that this course will be mainly an interactive demonstration at the microscope with only a limited amount of theory. The necessary theory for the course is covered in below mentioned online tutorials. Therefore, it is required to watch the following recommended tutorials in advance.

  1. Transmission light path and its alignment https://www.ibiology.org/talks/microscope-imaging-koehler-illumination/

  2. Fluorescence Microscopy https://www.ibiology.org/talks/fluorescence-microscopy/

  3. Cameras and Digital Image Analysis https://www.ibiology.org/talks/digital-image-analysis/

  4. Resolution of a Microscope: https://www.ibiology.org/talks/resolution-of-a-microscope/

  5. Objectives and optical aberrations (Objectives and Eyepieces)

Course program outline (additional breaks will be included on demand):

Estimated Time Topic
10:00 – 10:15 Welcome and introduction round
10:15 Р11:00 Transmission light path and Köhler illumination
11:00 – 12:30 Fluorescence microscopy, filters and how to find good filter fluorophore combinations
12:30 – 14:00 Lunch break
14:00 – 15:00 Microscope objectives (magnification, resolution and brightness) light Sources for epifluorescence
15:00 – 16:30 Cameras: types, exposure time, bit depth and LUT, background level, remarks on sample preparation
16:30 – 17:00 Discussion / Q&A and closing remarks

Further teaching material:

In case you are interested in learning the theory of widefield microscopy in more detail, please check out the following links:

  1. Olympus interactive Java tutorial https://www.olympus-lifescience.com/en/microscope-resource/primer/java/
  2. iBiology microscopy courses https://www.ibiology.org/online-biology-courses/microscopy-series/


Bookings are closed for this event.