Essentials of Widefield-Microscopy

almf   2020-01-20   Comments Off on Essentials of Widefield-Microscopy

Date/Time
Date(s) - 2020-01-20
13:00 CET - 18:00 CET

Categories


Essentials I – Essentials of Widefield-Microscopy

Please be aware, that this course will be mainly practical with only a limited amount of theory. Therefore, we strongly suggest to watch the following recommended tutorials in advance.

  1. Transmission light path and its alignment 
    https://www.ibiology.org/talks/microscope-imaging-koehler-illumination/

  2. Fluorescence Microscopy 
    https://www.ibiology.org/talks/fluorescence-microscopy/

Course program outline:

Time

Module

13:00 – 15:00

Practical – part 1

15:00 – 15:30

Break

15:30 – 17:30

Practical – part 2

17:30 – 18:00

Closing words, questions?

Practical part 1:

  • Transmission (Koehler, field diaphragm, condenser lens + aperture, sample, objective) 

  • Epi-light path (dichroic filters + filter wheel, excitation source)

  • How to find the right “filter fluorophore” combination

  • Challenges of a multicolor experiment

Practical part 2

  • sample prep, exposure time, excitation intensity, camera range 

  • Objective lenses (NA, magnification, background level)

Further teaching material:

In case you are interested in learning the theory of widefield microscopy in more detail, please check out the following links: 

  1. Olympus interactive Java tutorial 
    https://www.olympus-lifescience.com/en/microscope-resource/primer/java/

  2. iBiology microscopy courses 
    https://www.ibiology.org/online-biology-courses/microscopy-series/