RNAi tutorial and siRNA design

almf   2020-11-20   Comments Off on RNAi tutorial and siRNA design

Date/Time
Date(s) - 2020-11-20
09:30 CET - 15:30 CET

Categories


RNAi experiments and siRNA design

Morning – interactive tutorial ~ 2.5 h

Afternoon – hand-on session on siRNA design ~2 h

Course content:

  • RNAi reagents
  • General considerations of RNAi experiments
  • SiRNA transfection methods
  • Rescue / complementation experiments
  • Comparison CRISPR /RNAi
  • Open source siRNAs design tool
  • Introduction ENSEMBL database
  • Design of specific siRNAs against your gene of interest

In addition:

1. Please make sure that you have Firefox or Chrome as a browser. For some of the databases Internet Explorer is causing problems.

Web pages used:

<a
http://www.ensembl.org/Homo_sapiens/Info/Index
https://www.bioinformatics.org/sms/rev_comp.html
https://rnaidesigner.thermofisher.com/rnaiexpress/setOption.do?designOption=sirna&pid=-1981026287897157009

Bookings

Bookings are closed for this event.