Essentials I – Essentials of Widefield-Microscopy

Organizer: Stefan Terjung, Beate Neumann and Manuel Gunkel

Registration for EMBL staff only

The course is a beginners course with focus on teaching the essential knowledge needed to conduct a widefield microscopy-based experiment, to generate reliable data and to inspect and handle the acquired images. It will take place in the ALMF as a practical session on Monday October 17th (10 am – 5 pm). This course will be available also beginning of 2023.
Knowledge of this course will be the basis for the Essentials in Microscopy II (Confocal Microscopy) course.

For preparation of the course you will get a short list of videos covering the basics as well as a self-test questionaire

Topics covered include

• Transmission light path and Köhler illumination

• Fluorescence microscopy, filters and how to find good filter fluorophore combinations

• Light sources, Microscope objectives (magnification, resolution and brightness), Cover slips

• exposure time, bit depth and LUT, background level, remarks on sample preparation

Bookings