[ALMF]: Essentials II – Essentials of confocal microscopy

almf   2022-11-07   Comments Off on [ALMF]: Essentials II – Essentials of confocal microscopy

Date(s) - 2022-11-07
10:00 CET - 17:00 CET

Essentials II – Essentials of confocal microscopy

Organizer: Manuel Gunkel, Aliaksandr Halavatyi and Marko Lampe

 Registration for EMBL staff only 

The course is covering the essential knowledge needed to conduct confocal microscopy-based experiments. The course takes place as a practical in the ALMF on November 7th (10 am to 5 pm). This course will be available also beginning of 2023.

The prerequisite for attending this course is the knowledge covered in Essentials I course. The microscopy knowledge covered in this is mandatory for introductions into ALMF Confocal-microscopes.

For preparation of the course you will get a short list of videos covering the basics as well as a self-test questionaire

Topics covered include

  • Confocal principle
  • Optical sectioning
  • Confocal Lightpath
  • Scanning
  • Pinhole size
  • Lateral and axial resolution
  • 3D data acquisition with confocals
  • Saturation and offset
  • SNR Averaging/Accumulation, Voltage, laser power
  • Refractive index and spherical aberrations
  • Correcting for z-distortion
  • Multicolor imaging
  • Getting a transmitted light image with the confocal microscope
  • Sample types


Bookings are closed for this event.