Date(s) - 2023-01-16 - 2023-01-30
10:00 CET - 17:00 CET
Essentials I – Essentials of Widefield-Microscopy
Organizer: Stefan Terjung, Beate Neumann, Marko Lampe, Manuel Gunkel and Christian Tischer
Registration for EMBL staff only
The course is a beginners course with focus on teaching the essential knowledge needed to conduct a widefield microscopy-based experiment, to generate reliable data and to inspect and handle the acquired images. It will take place as a two day event and consists of a practical session on January 16th (10 am – 5 pm) in the ALMF and an image handling and inspection session on Wednesday January 30th (2 – 5 pm) together with CBA.
Knowledge of this course will be the basis for the Essentials in Microscopy II (Confocal Microscopy) course.
For preparation of the course you will get a short list of videos covering the basics as well as a self-test questionaire
Topics covered include
Transmission light path and Köhler illumination
Fluorescence microscopy, filters and how to find good filter fluorophore combinations
Light sources, Microscope objectives (magnification, resolution and brightness), Cover slips
exposure time, bit depth and LUT, background level, remarks on sample preparation
Data handling and ispection topics:
- Using ImageJ, we will cover the following topics related to 3D image data:
- Opening images
- Image content and inspection
- Image calibration
- Image data types
- Image file formats
This event is fully booked.