Date(s) - 2024-01-11
09:30 CET - 16:30 CET
Essentials I – Essentials of Widefield-Microscopy
Organizer: Marko Lampe, Beate Neumann, Stefan Terjung,
Registration for EMBL staff only
The course is a beginners course with focus on teaching the essential knowledge needed to conduct a widefield microscopy-based experiment and to generate reliable data It will take place as a one day event as a practical session on January 11th (9:30 am – 4:30 pm) in the ALMF.
Knowledge of this course will be the basis for the Essentials in Microscopy II (Confocal Microscopy) course.
For preparation of the course you will get a short list of videos covering the basics as well as a self-test questionaire
Topics covered include
Transmission light path and Köhler illumination
Fluorescence microscopy, filters and how to find good filter fluorophore combinations
Light sources, Microscope objectives (magnification, resolution and brightness), Cover slips
exposure time, bit depth and LUT, background level, remarks on sample preparation
This event is fully booked.