Basics in Optical Sectioning Microscopy (confocal, Deconvolution, TIRF, lightsheet)

Stefan Terjung   2019-02-12   Comments Off on Basics in Optical Sectioning Microscopy (confocal, Deconvolution, TIRF, lightsheet)
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Date/Time
Date(s) - 12/02/2019 - 15/02/2019
09:30 - 18:00

Location
ALMF, EMBL

Categories


ALMF in-house microscopy course part 2

Basics in Optical Sectioning Microscopy (confocal, lightsheet)

12 – 15 February 2019

Download course handouts, AIRY scan basic principle .

Our basic course 2 „Basics in Optical Sectioning Microscopy (confocal, Deconvolution, TIRF, lightsheet)” is intended for all interested people who are already familiar with the basics of light microscopy which were covered in the Basics in Light Microscopy course (including the basic concepts of light, the light path in a transmission and reflection microscope, fluorescence and terms like resolution, magnification, reflection …), but would like to understand the concepts of optical sectioning, with a focus on point scanning confocal microscopy.
The lectures of the course will cover the following topic:

  • Basic principal of confocality
  • Different types of confocal microscopes (point scanner, slit scanner, and spinning disc, two photon)
  • Different components of a confocal microscopes (lasers, detectors, acousto-optical elements, prisms …)
  • Optical sectioning
  • Confocal imaging parameters (pixel dwell; time signal to noise; pin hole size …)
  • Basic principal of Single Plane Illumination (SPIM) (different types of light sheets, geometry of SPIM systems, research example)
  • Ideal conditions for live cell imaging (environmental control, media, sample mounting, autofocus strategies, phototoxicity, imaging conditions)
  • Basic principal of Total Internal Reflection Fluorescence (TIRF)

During the practical’s we will apply the basic concepts learned during the lectures.

12 February
Lectures Room 202 (open for all)
9:30 – 12:30 Ulf Schwarz (Leica): Basics in Confocal Microscopy
online stream: https://tinyurl.com/BioIT-Stream,
please use your EMBL credentials to log on.
12:30 – 13:30 Lunch break
Hands-on : meeting point Room 13-502
14:00 – 18:00 Hands-on Confocal Microscopy

Instructors:  ALMF staff

13 February
Lectures Room 202 (open for all)
9:15 – 10:00 Sabine Reither: Live cell imaging
online stream: https://tinyurl.com/BioIT-Stream,
please use your EMBL credentials to log on.
10:00 – 10:45 Dimitri Kromm: Lightsheet Microscopy (SPIM)
online stream: https://tinyurl.com/BioIT-Stream,
please use your EMBL credentials to log on.
11:00 – 12:00 Time to attend the general assembly
14 February
Lectures Room 202 (open for all)
9:30 – 10:15 Marko Lampe: Basics in Total Internal Reflection Fluorescence (TIRF) Microscopy
online stream: https://tinyurl.com/BioIT-Stream,
please use your EMBL credentials to log on.
Hands-on meeting point Room 13-502
10:30 – 12:00 Advanced optical sectioning techniques (Spinning Disk , Deconvolution, AiryScan) cycle 1

Instructors: ALMF staff

12:00 – 14:00 Lunch break
14:00 – 17:00 Advanced optical sectioning techniques (Spinning Disk ,Deconvolution, AiryScan) cycle 2+3

Instructors: ALMF staff

15 February
Hands-on image processing groups A+B: Room 202
9:30 – 12:30 Hands-on image visualization

Instructor: Christian Tischer, CBA
online stream: https://tinyurl.com/BioIT-Stream,
please use your EMBL credentials to log on.

Bookings

Bookings are closed for this event.