RNAi tutorial and siRNA design

RNAi experiments and siRNA design

Morning – interactive tutorial ~ 2.5 h

Afternoon – hand-on session on siRNA design ~2 h

Course content:

• RNAi reagents
• General considerations of RNAi experiments
• SiRNA transfection methods
• Rescue / complementation experiments
• Comparison CRISPR /RNAi
• Open source siRNAs design tool
• Introduction ENSEMBL database
• Design of specific siRNAs against your gene of interest

In addition:

1. Please make sure that you have Firefox or Chrome as a browser. For some of the databases Internet Explorer is causing problems.

Web pages used:

<a
http://www.ensembl.org/Homo_sapiens/Info/Index
https://www.bioinformatics.org/sms/rev_comp.html
https://rnaidesigner.thermofisher.com/rnaiexpress/setOption.do?designOption=sirna&pid=-1981026287897157009

Bookings