RNAi experiments and siRNA design
Morning – interactive tutorial ~ 2.5 h
Afternoon – hand-on session on siRNA design ~2 h
Course content:
- RNAi reagents
- General considerations of RNAi experiments
- SiRNA transfection methods
- Rescue / complementation experiments
- Comparison CRISPR /RNAi
- Open source siRNAs design tool
- Introduction ENSEMBL database
- Design of siRNAs using publicly available tools
In addition:
1. Please make sure that you have Firefox or Chrome as a browser. For some of the databases Internet Explorer is causing problems.
We will use the following webpages:
<a
href=”http://www.ensembl.org/Homo_sapiens/Info/Index”>http://www.ensembl.org/Homo_sapiens/Info/Index
https://www.bioinformatics.org/sms/rev_comp.html
https://rnaidesigner.thermofisher.com/rnaiexpress/setOption.do?designOption=sirna&pid=-1981026287897157009
Bookings
Bookings are closed for this event.