Essentials II – Essentials of confocal microscopy

Organizer: Manuel Gunkel, Aliaksandr Halavatyi and Marko Lampe

 Registration for EMBL staff only 

The course is covering the essential knowledge needed to conduct confocal microscopy-based experiments. The course takes place as a practical in the ALMF on November 7th (10 am to 5 pm). This course will be available also beginning of 2023.

The prerequisite for attending this course is the knowledge covered in Essentials I course. The microscopy knowledge covered in this is mandatory for introductions into ALMF Confocal-microscopes.

For preparation of the course you will get a short list of videos covering the basics as well as a self-test questionaire

Topics covered include

• Confocal principle
• Optical sectioning
• Confocal Lightpath
• Scanning
• Pinhole size
• Lateral and axial resolution
• 3D data acquisition with confocals
• Saturation and offset
• SNR Averaging/Accumulation, Voltage, laser power
• Refractive index and spherical aberrations
• Correcting for z-distortion
• Multicolor imaging
• Getting a transmitted light image with the confocal microscope
• Sample types

Bookings