Date(s) - 2022-10-17
10:00 CEST - 17:00 CEST
Essentials I – Essentials of Widefield-Microscopy
Organizer: Stefan Terjung, Beate Neumann and Manuel Gunkel
Registration for EMBL staff only
The course is a beginners course with focus on teaching the essential knowledge needed to conduct a widefield microscopy-based experiment, to generate reliable data and to inspect and handle the acquired images. It will take place in the ALMF as a practical session on Monday October 17th (10 am – 5 pm). This course will be available also beginning of 2023.
Knowledge of this course will be the basis for the Essentials in Microscopy II (Confocal Microscopy) course.
For preparation of the course you will get a short list of videos covering the basics as well as a self-test questionaire
Topics covered include
Transmission light path and Köhler illumination
Fluorescence microscopy, filters and how to find good filter fluorophore combinations
Light sources, Microscope objectives (magnification, resolution and brightness), Cover slips
exposure time, bit depth and LUT, background level, remarks on sample preparation
This event is fully booked.