Essentials I – Essentials of Widefield-Microscopy
Organizer: Beate Neumann, Stefan Terjung,
Registration for EMBL staff only
The course is a beginners course with focus on teaching the essential knowledge needed to conduct a widefield microscopy-based experiment and to generate reliable data It will take place as a one day event as a practical session on January 13th (9:30 am – 5 pm) in the ALMF.
Knowledge of this course will be the basis for the Essentials in Microscopy II (Confocal Microscopy) course.
For preparation of the course you will get a short list of videos covering the basics as well as a self-test questionaire
Topics covered include
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Transmission light path and Köhler illumination
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Fluorescence microscopy, filters and how to find good filter fluorophore combinations
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Light sources, Microscope objectives (magnification, resolution and brightness), Cover slips
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exposure time, bit depth and LUT, background level, remarks on sample preparation