Date(s) - 2021-05-07
10:00 CEST - 17:00 CEST
Essentials I – Essentials of Widefield-Microscopy (online)
Organizer: Sabine Reither, Lucas Schuetz and Faba (Beate) Neumann
Registration for EMBL staff only
The course is a beginners course with focus on teaching the essential knowledge needed to conduct a widefield microscopy-based experiment and to generate reliable data. Knowledge of this course will be the basis for the Essentials in Microscopy II (Confocal Microscopy) course.
Please be aware that this course will be mainly an interactive demonstration at the microscope with only a limited amount of theory. The necessary theory for the course is covered in below mentioned online tutorials. Therefore, it is required to watch the following tutorials in advance.
Course program outline (additional breaks will be included on demand):
|10:00 – 10:15||Welcome and introduction round|
|10:15 – 11:00||Transmission light path and Köhler illumination|
|11:00 – 12:30||Fluorescence microscopy, filters and how to find good filter fluorophore combinations|
|12:30 – 14:00||Lunch break|
|14:00 – 15:00||Light sources, Microscope objectives (magnification, resolution and brightness), Cover slips|
|15:00 – 16:30||What is a good image: exposure time, bit depth and LUT, background level, remarks on sample preparation|
|16:30 – 17:00||Discussion / Q&A and closing remarks|
Further teaching material:
In case you are interested in learning the theory of widefield microscopy in more detail, please check out the following links:
- Olympus interactive Java tutorial https://www.olympus-lifescience.com/en/microscope-resource/primer/java/
- iBiology microscopy courses https://www.ibiology.org/online-biology-courses/microscopy-series/
- Microcourse done by Jennifer Waters https://www.youtube.com/channel/UC4cOKa0TZK8CQhzSQqfwuMQ/
This event is fully booked.