[ALMF]: Essentials II – Essentials of confocal microscopy (online)

almf   2021-05-10   Comments Off on [ALMF]: Essentials II – Essentials of confocal microscopy (online)

Date/Time
Date(s) - 2021-05-10
10:00 CEST - 17:00 CEST

Categories

Essentials II – Essentials of confocal microscopy (online)

Organizer: Manuel Gunkel, Aliaksandr Halavatyi and Marko Lampe

 Registration for EMBL staff only 

The course is covering the essential knowledge needed to conduct a confocal microscopy-based experiments. Along with that, we discuss advanced topics of microscopy and fluorescence imaging.The prerequisite for attending this course is the knowledge covered in Essentials I course. The microscopy knowledge covered in this is mandatory for introductions into ALMF Confocal-microscopes.

Please be aware, that this course mainly cover practical aspects with only a limited amount of theory. Therefore, we strongly suggest to watch the following recommended tutorials in advance.

Course outline

Practical point scanning confocal – part 1:

  1. Why/when one needs optical sectioning
  2. Lightpath
  3. Why need to scan
  4. Confocal principle
  5. Pinhole size
  6. 3D data acquisition with confocals

Practical point scanning confocal – part 2:

  1. Lateral and axial resolution
  2. Selecting pixel size
  3. Choosing an objective
  4. Saturation and offset
  5. SNR Averaging/Accumulation, Voltage, laser power

Practical point scanning confocal – part 3:

  1. How to achieve higher frame rates
  2. 3D proer stack configuration
  3. XZ scanning
  4. Refractive index and spherical aberrations
  5. Correcting for z-distortion
  6. Minimizing spherical aberrations

Practical point scanning confocal – part 4:

  1. Multicolor imaging – use of sequences and tracks
  2. Multicolor imaging – configuration of detectors
  3. Chromatic aberrations
  4. Point scanning multicolor imaging – spectral detectors
  5. Getting transmitted light image
  6. Sample types

Q&A session

Bookings

This event is fully booked.