Essentials II – Essentials of confocal microscopy

Organizer: Manuel Gunkel, Aliaksandr Halavatyi

Registration for EMBL staff only

The course covers the essential knowledge needed to conduct confocal microscopy-based experiments and takes place in the ALMF on January 23rd (9:30 am to 4:30 pm).
The prerequisite for attending this course is the knowledge covered in the Essentials I course. The microscopy knowledge covered in this is mandatory for introductions into ALMF Confocal-microscopes.

For preparation of the course you will get a short list of videos covering the basics.

Topics covered include:

• Confocal principle
• Optical sectioning
• Confocal Lightpath
• Scanning
• Pinhole size
• Lateral and axial resolution
• 3D data acquisition with confocals
• Saturation and offset
• SNR Averaging/Accumulation, Voltage, laser power
• Refractive index and spherical aberrations
• Correcting for z-distortion
• Multicolor imaging
• Getting a transmitted light image with the confocal microscope
• Sample types

Bookings