Essentials I – Essentials of Widefield-Microscopy (online)

Fully booked, please visit https://bio-it.embl.de/events/essentials-i-essentials-of-widefield-microscopy-online-2/ for alternative date

Organizer: Sabine Reither, Marko Lampe and Lucas Schuetz

The course is a beginners course with focus on teaching the essential knowledge needed to conduct a widefield microscopy-based experiment and to generate reliable data. Knowledge of this course will be the basis for the Essentials in Microscopy II (Confocal Microscopy) course.

Please be aware that this course will be mainly an interactive demonstration at the microscope with only a limited amount of theory. The necessary theory for the course is covered in below mentioned online tutorials. Therefore, it is required to watch the following recommended tutorials in advance.

Transmission light path and its alignment https://www.ibiology.org/talks/microscope-imaging-koehler-illumination/
Fluorescence Microscopy https://www.ibiology.org/talks/fluorescence-microscopy/
Cameras and Digital Image Analysis https://www.ibiology.org/talks/digital-image-analysis/
Resolution of a Microscope: https://www.ibiology.org/talks/resolution-of-a-microscope/
Objectives and optical aberrations (Objectives and Eyepieces)

Course program outline:

Estimated Time	Topic
13:00 – 13:15	Welcome and introduction round
13:15 – 14:00	Transmission light path and Köhler illumination
14:00 – 14:10	Break
14:10 – 15:00	Fluorescence microscopy, Filters and how to find good filter fluorophore combinations
15:00 – 15:15	Break
15:15 – 16:00	Microscope Objectives (Magnification, resolution and brightness)Light Sources for Epifluorescence
16:00 – 16:15	Break
16:15 – 17:00	Cameras: types, exposure time, bit depth and LUT, background level, Remarks on sample preparation
17:00 – 17:10	Break
17:10 – 17:45	Discussion / Q&A
17:45 – 18:00	Closing remarks

Further teaching material:

In case you are interested in learning the theory of widefield microscopy in more detail, please check out the following links:

Olympus interactive Java tutorial https://www.olympus-lifescience.com/en/microscope-resource/primer/java/
iBiology microscopy courses https://www.ibiology.org/online-biology-courses/microscopy-series/

Bookings

This event is fully booked.