Essentials of Confocal-Microscopy

almf   2020-03-02   Comments Off on Essentials of Confocal-Microscopy

Date/Time
Date(s) - 02/03/2020
13:00 - 18:30

Categories


Essentials of Confocal-Microscopy 

Please be aware, that this course will be mainly practical with only a limited amount of theory. Therefore, we strongly suggest to watch the following recommended tutorials in advance.

Teaching material:

  1. Basics of confocal microscopy 
    https://www.ibiology.org/talks/confocal-microscopy/

  2. Nikon Microscopy University – Introductory Confocal Concepts 
    https://www.microscopyu.com/techniques/confocal/introductory-confocal-concepts

Course program outline:

Time

Module

13:00 – 13:30

Introduction to practical sessions

13:30 – 15:15

Practical point scanning confocal – part 1

15:15 – 15:30

Break

15:30 – 17:00

Practical point scanning confocal – part 2

17:00 – 18:30

Q&A session and overview of advanced optical sectioning techniques available in ALMF

Practical part

Practical part 1:

  1. Why/when one needs optical sectioning

  2. lightpath

  3. why need to scan

  4. confocal principle

  5. pinhole size

  6. lateral and axial resolution

  7. selecting pixel size

  8. multicolor imaging – use of sequences and tracks and configuration of detectors

Practical part 2

  1. saturation and offset

  2. how to achieve higher frame rates 

  3. getting transmitted light image Point scanning confocal

  4. 3D data acquisitions with confocals

Q&A session:

  1. Alternatives to point scanning confocal  (TIRF, spinning disk, multiphoton, AiryScan, SPIM)

Bookings

This event is fully booked.